METU MESOCOSM SYSTEM 2019
Aquatic research is still divided in marine and freshwater sciences, with separate funding sources, and experimental science is often conducted at single sites without international coordination. To challenge these problems, the FvB-IGB-lead project AQUACOSM is funded by the first international call (EU H2020-INFRAIA) to coordinate research, develop common best practices and open both freshwater and marine large-scale research infrastructures (mesocosms) for international cross-discipline participation
Project Title: EU H2020-INFRAIA-project No 731065 "AQUACOSM: Network of Leading European AQUAtic MesoCOSM Facilities Connecting Mountains to Oceans from the Arctic to the Mediterranean"
Funded by: European Commission, Directorate-General for Research & Innovation, Budget 9,999,807 €
Project partners: Aarhus University, AREFSENS Electronics Inc., Blue Lobster IT Ltd, Centro de Biodiversidade e Recursos Genéticos – Universidade de Évora, CNRS – MARine Biodiversity-Exploitation and Conservation, Ecole Normale Supérieure, Finnish Environment Institute-Marine Research Centre, Forschungsverbund Berlin e.V. – Leibniz-Institut für Gewässerökologie und Binnenfischerei, GEOMAR Helmholtz Centre for Ocean Research Kiel, Hellenic Centre for Marine Research, Imperial College of Science Technology and Medicine, Ludwig-Maximilians-Universität, Middle East Technical University, Netherlands Institute of Ecology, Norwegian Institute for Water Research, Umeå Marine Science Center, Umweltbundesamt, Uni Research AS, University of Bergen, University of Helsinki-Tvärminne Zoological Station, WasserCluster Lunz-Biologische Station GmbH
As a partner of AQUACOSM: the EU-network connecting freshwater and marine large experimental research infrastructures, METU MESOCOSM SYSTEM hosts researchers worldwide and together they conduct research on the impacts of climate change in freshwater ecosystem using METU Mesocosm System. For more information on the AQUACOSM project, please visit the project website at https://www.aquacosm.eu/.
METU MESOCOSM SYSTEM 2019 PROJECT:
Dissolved Organic Carbon(DOC)– Impacts of Pulsed DOC Disturbance in shallow freshwater lakes
Time and Location
The 2019 mesocosm experiment was conducted at METU Mesocosm System at METU Experimental Lake (Lake Golet) between 20 June – 26 July 2019.
The primary objective of the experiment is to investigate the responses of the system to dissolved organic carbon (DOC) pulses from terrestrial sources as a part of Joint mesocosm experiments of Aquacosm (JOMEX). Through the changes in precipitation pattern in the eastern Mediterranean lately, winter snow packs have been largely replaced with early and late spring heavy rains and intensive flush floods. Such conditions increase transport of matter from terrestrial environments into aquatic systems is a highly relevant aspect of global change even in Eastern Mediterranean in Turkey
We investigated the response of different plankton communities to a pulsed DOC disturbance and followed the reaction of the system after this strong disturbance in terms of resistance, resilience and recovery (i.e. ecosystem stability concept). We hypothesized that 1) DOC will cause compositional and/or functional shifts in the communities following resistance, resilience, recovery stages, 2) the effects of these shifts will be transferred throughout the food web, 3) systems with high initial functional diversity will have a faster recovery towards initial conditions
For this purpose, METU Mesocosm System at Lake Golet was used with 16 mesocosms with 4x4 design. In order to simulate the effects of terrestrial DOC, two different DOC source was tested: (i) Local DOC extracted from Local Alder Tree Leaves to test the nutrient effect of terrestrial DOC on freshwater systems (ii) Humin Feed (Humintech GmbH), which is a highly standardized product that is used to test light limitation effect of DOC on freshwater systems. Therefore, Mesocosm System included 16 mesocosms with 4 Controls, 4 replicates including a highly standardized DOC source (4 ppm HF) and 4 replicates including a local, more site-specific DOC source (8 ppm Local DOC) and 4 replicates including both sources (4 ppm HF and 8 ppm Local DOC).
With the aim of understanding whole-ecosystem responses, we investigated physiochemical parameters, microbial community structure (16S-18S rRNA), microbial loop community dynamics (bacteria, HNF, ciliates), phytoplankton and zooplankton community structure, lake metabolism and lake stoichiometry.
We had TA Access teams with projects MicroMESO, PperDOC, ECMMETU2019 and AlloEcoMetry, comprising scientists from Greece, Turkey, Brazil, Croatia and Ireland.
Assist. Prof. Dr. Emmanuel (Manolis) LADOUKAKIS, Department of Biology, University of Crete, Greece
Assoc. Prof. Dr. Efe Sezgin, Department of Food Engineering , Izmir Institute of Technology
PhD student Marilena Parakatselaki, Department of Biology, University of Crete, Greece
MSc student Hüseyin Yiğit Şahin, Ege University & Aegene Biotechnology Inc., Turkey
Dr. Kemal Ali Ger, Universidade Federal do Rio Grande do Sul-UFRGS, Brazil
Prof. Dr. Maria Spoljar, Department of Biology, University of Zagreb, Croatia
Prof. Dr. Sanja Gottstein, Department of Biology, University of Zagreb, Croatia
MSc Student Claudia Fiorentin, Department of Biology, University of Zagreb, Croatia
Dr. Mikkel Rene Anderson, Dundalk Institute of Technology, Ireland
Research Assistant Pınar Kavak Gulbeyaz, Department of Environmental Engineering, Hacettepe University, Turkey
Prof. Dr. Nusret Karakaya, Department of Environmental Engineering, Bolu Abant Izzet Baysal University, Turkey
PhD student Maria Caldero Pascual, Dundalk Institute of Technology, Ireland